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Production of Bioproducts for Industrial Applications
Team:
Prof. Taha Abdallah Kumosani 1
Prof. Saleh Ahmed Mohamed 1
Prof. Youssry Mohamed Soliman 1
Prof. Mahmoud Abbas Saleh 2
Prof. Elie Kamil Barbour 3
Prof. Ibrahim Hasan Ibrahim 1
Prof. Steve Mostafa Harakeh 4
Dr. Archana Panchapakesan Iyer 1
Dr. Mazen Abdulaziz Zamzami 1
Dr. Osama Mohamed Ashour Mohamed 5
Dr. Sawsan Omer Khoja 1
Dr. Khadiga Saead Balamish 1
Dr. Soonham Sami Yaghmoor 4
Dr. Jehad Mostafa Yousef 6
Dr. Wedad Makhdour AlBashri 6
1 Biochemistry Dept., Faculty of Science
2 Adjunct prof in Biochemistry Dept., Faculty of Science and Prof of Texas Southern University, USA.
3 Adjunct prof in Biochemistry Dept., Faculty of Science and Prof of American University of Beirut, Lebanon.
4 King Fahd Medical Research Center
5 Department of Pharmacology and Toxicology
6 Biochemistry Dept., Faculty of Science, Faisaliah branch
Summary:
Production of bioproducts for industrial applications is defined as the technology where living cells or their components and enzymes are used for the manufacture of industrial products or services. The research group will be focused on: a) Production and improvement of enzymes such as lipase, amylases, acylases, proteinases, cellulases and isomerases for industrial applications, b) The development of biotechnological processes for product manufacture of bulk industrial organic acids such as citric acid, lactic acid and itaconic acid, which used in food and pharmaceutical industries, c) The development of processes for production of biosurfactants for the detergent, healthcare and petroleum industries, d) Production of essential amino acid especially for the animal feed and aquaculture industries.
The findings of the research group:
The purification and characterization of M. racemsus lipase was studied. The purified lipase was formulated in the carbopol 934 gel and was utilized for topical treatment of cellulite, and the results were positive for reducing thigh circumference (Mohamed et al., 2011a).
The notion that a targeted antioxidant prophylaxis may be created using immobilized catalase (CAT) is extremely important. This innovative work demonstrated the feasibility of the preparation of flexible biocompatible nanosugar ester vesicles. The encapsulation of CAT in the vesicles enhanced its kinetic parameters, pH, thermal stability, reusability and protection against trypsin. New opportunities for the topical application of enzymatic radical scavengers CAT-EV in post-burn damage were confirmed in vivo. In conclusion, the flexible nanosugar ester vesicles developed in this study are a novel addition to the clinical and industrial arsenal for safe and active CAT topical photoprotective applications (Abdel-Mageed et al., 2012).
α-Amylase activity was screened in the peel, as waste fruit, of 13 species and cultivars of Egyptian citrus. The species Citrus sinensis cv. Abosora had the highest activity. The results suggested that, in addition to citrus peel is a rich source of pectins and flavanoids, α-amylase from orange peel could be involved in the development and ripening of citrus fruit and may be used for juice processing (Mohamed et al., 2010).
The production, purification and characterization of α-amylase from Trichoderma harzianum grown on mandarin peel were investigated. These properties of α-amylase with its remarkable activity meet the prerequisites needed for liquefaction and saccharification of starch industry (Mohamed et al., 2011).
The purification and characterization of peroxidase from miswak was studied. In addition, the study reported that miswak peroxidase was more stable to the denaturation induced by pH, heat, metal ions, metal chelators and proteolytic activity. The enzyme oxidized some of phenolic compounds. In the future, we will study the other antioxidant and digestive enzymes in miskwak for using of these enzymes in construction of a potent toothpast (Mohamed et al., 2012).
The characterization of peroxidase of citrus show that the instability of peroxidase above 50°C makes the high temperature short time treatment very efficient for the inactivation of peel peroxidase contaminated in orange juice to avoid the formation of off-flavors (Mohamed et al., 2008).
The enhaced L-glutamic acid production by wild type and mutants of Corynebacterium sp. grown on date syrup as carbon source was performed and the effect of some culture conditions was evaluated (Ahmed et al., 2012).
Vision of the research group:
To spearhead the development of Production of bioproducts for industrial applications which are of significant importance to Saudi Arabia.
Mission of the research group:
Establishment of production of bioproducts for industrial applications.
General Objectives:
1. To promote the establishment of production of bioproducts for industrial applications.
2. To plan and implement capacity development activities to ensure the availability of skilled personel required for biotechnology industries.
3. To coordinate and spearhead research and development activities toward commercialisation to achieve the targeted goal.
Specific Objectives:
1. Screening and isolation of indigenous microbial strain.
2. Development of bioreactors and fermentation process optimal for the microorganism and the production of bioproducts.
3. Development of appropriate downstream processing technique for separation and purification of the bioproducts.
4. Field trial to test for product efficiency and economic.
Suggested Researches by the group:
1. Production of enzymes for industrial applications.
2. Production of biosurfactants for petroleum industries.
The Importance of the Work of Proposed Research Group to the University and the Society:
1. Enhancing cooperation between different departments of the university
2. Stimulate creative thinking and facilitate collaborative research.
3. Planned activities will facilitate active integration of proposed group with community, organizations and institutions interested in these aspects.
The Expected Outcome:
This proposal will be produced:
- High volume, intermediate-value commodities (e.g. organic acids, vitamins, amino acids, food products).
- Low volume, high-value products (e.g. pharmaceuticals, enzymes or speciality chemicals).
Consultant:
Prof. Dr. rer. Nat. Hesham Ali El-Enshasy
Assistant Director for Innovation and Bioproducts Development
Institute of Bioproducts Development
Universiti Teknologi Malaysia (UTM)
81310 Skudai, Johor, Malaysia
E-mail: henshasy@lojipandu.utm.my
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